I've thought about selling it but taking it apart and sorting it sounds like a nightmare. Do you think you or anyone would actually buy that set pre-built, or is it pointless without the fun of building it?
The extension of microscale thermophoresis (MST) to perform temperature jumps by increasing the heat dissipation of the sample allows for quantification of binding affinities together with the kinetic rates. Relaxation kinetics for DNA hybridization were measured with high fidelity at different temperatures and various experimental conditions. The results shed light on the hybridization mechanism of DNA and confirmed determinants of DNA binding.
Microscale thermophoresis (MST) is a versatile technique to measure binding affinities of binder–ligand systems, based on the directional movement of molecules in a temperature gradient. We extended MST to measure binding kinetics as well as binding affinity in a single experiment by increasing the thermal dissipation of the sample. The kinetic relaxation fingerprints were derived from the fluorescence changes during thermodynamic re-equilibration of the sample after local heating. Using this method, we measured DNA hybridization on-rates and off-rates in the range 104–106 m−1 s−1 and 10−4–10−1 s−1, respectively. We observed the expected exponential dependence of the DNA hybridization off-rates on salt concentration, strand length and inverse temperature. The measured on-rates showed a linear dependence on salt concentration and weak dependence on strand length and temperature. For biomolecular interactions with large enthalpic contributions, the kinetic MST technique offers a robust, cost-effective and immobilization-free determination of kinetic rates and binding affinity simultaneously, even in crowded solutions.