FERMENTED SEAWEED (Kappaphycus alverezii) BY-PRODUCT PROMOTES GROWTH AND DEVELOPMENT OF LETTUCE (Lactuca sativa VAR. CURLY GREEN) | PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY

Seaweed processing into juice extraction yields a considerable volume by product, with 800 grammes (80 percent) of residue going to waste following the extraction from a kilo of seaweed (Kappaphycus alverezii). The goal of this study was to employ Fermented Marine Macro Algae Residue to boost lettuce growth and yield in terms of height, growth rate, leaf length, and above and below ground fresh weight. A commercially available seaweed-based fertiliser (10ml/L) was compared to solid seaweed sludge (by-product of fermentation at 10tons/ha), fermented liquid seaweed (10ml/L), and a mix of both. Plant height after five days following transplanting for lettuce (Lactuca sativa var. Curly Green) indicated that seaweed sludge (solid seaweed fertiliser) outperformed commercial fertiliser at 10.010.72SE cm and 6.280.0.57SE cm, respectively. On the 20th day, the plants with the tallest plants (plant height) and the longest leaves (13.870.42SE cm and 15.430.54SE cm, respectively) were created using a mixture of liquid and solid seaweed fertiliser. With a combination application of liquid and solid seaweed fertiliser at 5.310.444SE cm, the fastest growth rate was achieved between 16 and 20 days. The highest growth rate was achieved by using solid seaweed fertiliser alone from transplanting (0-5 days) (4.080.44SE cm/5-days). Liquid fertiliser application resulted in almost three times larger above ground weight (270.17163.61SE g/plant) than all other treatments with equivalent below ground weight. As a result, waste products from the processing of seaweed-based beverages can be used as fertiliser in both liquid and solid form, and more significantly, in combination, for the production of lettuce.

Please see the link :- https://ikprress.org/index.php/PCBMB/article/view/5803

Keywords – Seaweed, organic agriculture, fermentation, sludge, liquid fertilizer

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Time to put the Lactuca sativa in the ground.
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You guys might enjoy the color of Aloe capitata var quartziticola
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Young Aloe capitata var. quartziticola showing off some color.
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how many kb can this baby fit?

tl;Dr: I'm trying to get a whole metabolic pathway into lettuce (Lactuca Sativa) via agrobacterium transformation. The pathway isn't the issue, but I'm trying to find out how many kb of DNA I can put into the caset and still get good results from the transformation?

Hi there! I'm writing a proposal for funding for an experiment where the end goal is a cultivar of red lettuce (Lactuca Sativa) which is able to produce ergosterol, or pro vitamin D. There are tons of mushrooms that make this compound, however this is a 21+ step enzymatic reaction. In stead of trying to ID the genes in the fungi of choice and then optimizing the codons from there I was thinking of taking the sequence of the enzymes, turning those back into DNA while choosing a more optimized codon bias, and then adding exons as needed. Once that is done I was going to have a caset made with CMV promotor to be grown in agrobacterium and then use them to transform the lettuce. Only problem is I know 21+ enzymes worth of DNA is going to be massive, and I don't know how I'll fit all of that into a caset. I was already thinking of doing multiple transformations but how many would depend on how big the caset can be and that really depends on how much DNA can successfully be integrated into the lettuce and THATS where I need your help! Also, if you have anymore pointers on experiment design, materials, or methods I am all ears! This is my very first project I get to take the lead on and it feels like I'm driving a rocket ship! Thank you tons for your help :)

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[Artemisia dracunculus var. sativa] French Tarragon - always wash fresh herbs. [1009x757]
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hiç esrar kullandınız mı ?
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12 seeds of landrace β€œSinai,” planted in 50/50 coco/compost today. From Mount Sinai in Egypt, where it is said Moses received the 10 commandments from an omnipresent spirit who went by many names including Yahweh, Elohim and El Shaddai. Fingers crossed this is the bush that was burning that day. 🀞 reddit.com/gallery/w0k9d1
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NFT flow rate question.

The general consensus for nft flow rate is 1 to 2 l per minute, however I recently just read a paper stating the best flow rate found for growth(lettuce) was .3L liters a minute, which is drastically different than the norm. The study stated slowing down the flow rate allows more contact time for the roots to pull the nutrients out of the water.

Does anybody on here run less than the recommended 1-2l /min?

https://www.researchgate.net/publication/328532184_Effect_of_water_flow_rate_on_quantity_and_quality_of_lettuce_Lactuca_sativa_L_in_nutrient_film_technique_NFT_under_hydroponics_conditions

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Anyone going to Lettuce tonight with extra space in their car for one?

My ride just bailed and my car is in the shop. Thanks for any help!

EDIT: Thanks for the upvotes!

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Which primers do I need to fingerprint lettuce? Help needed

I have a research competence for school, but to progress forward I need to find out what DNA primers I would need for the PCR reaction I'm about to do with 4 different lettuce crops.

The lettuce crops in question are:

  • Butter lettuce (Lactuva sativa var. capitata)
  • Iceberg lettuce (Lactuva sativa)
  • Wild Lettuce (Lactuva serriola)
  • Stem Lettuce (Lactuva sativa var. angustana)

On a paper I found online, it indicated Er1Bh1Blunt as a primer, but I'm not completely sure.

Any help will be appreciated, it is for a big part of my points this semester. Thank you in advance!!

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Romaine Lettuce - it really works (for me at least)

I've been using Lactuca virosa (wild lettuce) tincture every now and then before bed - not only as a sedative/analgesic but also as a profound oneirogen/dream enhancer, for which the main compound responsible is likely lactucopicrin, an acetylcholinesterase inhibitor. While our understanding of the role of acetylcholine in REM sleep and dreaming is primitive, galantamine, a pharmaceutical acetylcholinesterase inhibitor, is known to induce vivid dreams and has been mentioned on this sub a few times. Lactucopicrin has been found in many lettuce species including Lactuca sativa, the stuff you buy at the grocery store.

Despite the lower concentration, eating a few leaves of romaine lettuce 30 minutes or so before sleep has made lucid dreaming much eaiser in my recent experience. I've achieved lucidity the majority of nights I've tried this, which normally isn't the case for me. Whether my sample size is too small or there's some implicit bias where I try harder to lucid dream on the lettuce nights I can't say, but I'm encouraging people to try this and share their experiences.

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