Cannabidiol protects keratinocyte cell membranes following exposure to UVB and hydrogen peroxide [2020] sciencedirect.com/science…
πŸ‘︎ 28
πŸ“°︎ r/DrugNerds
πŸ’¬︎
πŸ“…︎ Sep 14 2020
🚨︎ report
Novel cannabidiol sunscreen protects keratinocytes and melanocytes against ultraviolet B radiation [2020] onlinelibrary.wiley.com/d…
πŸ‘︎ 9
πŸ“°︎ r/DrugNerds
πŸ’¬︎
πŸ“…︎ Sep 25 2020
🚨︎ report
2020 ap biology exam frataxin and aquaporins/keratinocytes

Can someone also start a discussion trend for this plzzzz

πŸ‘︎ 6
πŸ“°︎ r/APbio
πŸ’¬︎
πŸ“…︎ May 19 2020
🚨︎ report
5-aminolaevulinic acid-based photodynamic therapy induces both necrosis and apoptosis of keratinocytes in plantar warts tandfonline.com/doi/abs/1…
πŸ‘︎ 3
πŸ“°︎ r/HPV
πŸ’¬︎
πŸ‘€︎ u/xdhpv
πŸ“…︎ Jul 04 2020
🚨︎ report
FXN and aquaporin/keratinocytes

Anyone get that question that said what would happen to frataxin production in mice with fff, all lowercase? Did you say an increase or decrease would occur because I’m still lost.

πŸ‘︎ 2
πŸ“°︎ r/APbio
πŸ’¬︎
πŸ“…︎ May 19 2020
🚨︎ report
Has anyone heard of hair regrowth peptide and vitamin combo US9700504b2. It’s a combo of fibroblast growth factor, epithelial growth, follistatin plus Wnt protein, keratinocyte growth factor, IGF-1. It supposedly has a patent. Does anyone have any idea what this does and if it works?
πŸ‘︎ 6
πŸ“°︎ r/hairgrowth
πŸ’¬︎
πŸ‘€︎ u/kambian669
πŸ“…︎ May 11 2020
🚨︎ report
How do you beat a cell protected by keratinocytes?

So I designed a few species of

flagellocyte-phagocyte-keratinocyte-hostile pattern

I want to know how best to design a species to be able to compete with them

πŸ‘︎ 6
πŸ“°︎ r/CellLab
πŸ’¬︎
πŸ‘€︎ u/DataScavenger
πŸ“…︎ Mar 03 2020
🚨︎ report
High glucose augments ROS generation regulates mitochondrial dysfunction and apoptosis via stress signalling cascades in keratinocytes. - December 2019

https://www.ncbi.nlm.nih.gov/pubmed/31830478

Rizwan H1, Pal S1, Sabnam S1, Pal A2.

Abstract

Mitochondria are fascinating structures of the cellular compartments that generate energy to run the cells. However, inherent disorders of mitochondria due to diabetes can cause major disruption of metabolism that produces huge amount of reactive oxygen species (ROS). Here we study the elevated level of ROS provoked by high glucose (HG) environment triggered mitochondrial dysfunction, inflammatory response and apoptosis via stress signalling pathway in keratinocytes. Our results demonstrated that elevated glucose level in keratinoctes, increase the accumulations of ROS and decrease in cellular antioxidant capacities. Moreover, excess production of ROS was associated with mitochondrial dysfunction, characterized by loss of mitochondrial membrane potential (ΔΨm), increase in mitochondrial mass, alteration of mitochondrial respiratory complexes, cytochrome c (Cyt c) release, decrease in mitochondrial transcription factor A (TFAM) and increase in mitochondrial DNA (mtDNA) fragmentation. Damaged mtDNA escaped into the cytosol, where it engaged the activation of ERK1/2, PI3K/Akt, tuberin and mTOR via cGAS-STING leading to IRF3 activation. Pre-treatment of pharmacological inhibitors, ERK1/2 or PI3K/Akt suppressed the IRF3 activation. Furthermore, our results demonstrated that activation of IRF3 in HG environment coinciding with increased expression of inflammatory mediators. Excess production of ROS interfered with decreased in cell viability, increased lysosomal content and expression of FoxOs, leading to cell cycle deregulation and apoptosis. Pre-treatment of N-acetyl-l-cysteine (NAC) significantly reduced the HG-induced cell cycle deregulation and apoptosis in keratinocytes. In conclusion, increased oxidative stress underlies the decrease in antioxidant capacities and mitochondrial dysfunction in HG environment correlate with inflammation response and apoptosis via ERK1/2-PI3K/Akt-IRF3 p

... keep reading on reddit ➑

πŸ‘︎ 56
πŸ“°︎ r/ketoscience
πŸ’¬︎
πŸ‘€︎ u/Ricosss
πŸ“…︎ Dec 17 2019
🚨︎ report
Stunning video of a live Keratinocyte and its organelles at super resolution v.redd.it/wkv405m6jxl31
πŸ‘︎ 938
πŸ“°︎ r/sciences
πŸ’¬︎
πŸ‘€︎ u/pollisa_85
πŸ“…︎ Sep 11 2019
🚨︎ report
Stunning video of a live Keratinocyte and its organelles at super resolution v.redd.it/rujcm31zjxl31
πŸ‘︎ 60
πŸ“°︎ r/microscopy
πŸ’¬︎
πŸ‘€︎ u/pollisa_85
πŸ“…︎ Sep 11 2019
🚨︎ report
MALT1 protease activity as a new pharmacological target against psoriasis demonstrated in a mouse model (keratinocyte-specific inducible expression of a psoriasis-associated mutant gene). embopress.org/doi/abs/10.…
πŸ‘︎ 2
πŸ“°︎ r/regenerate
πŸ’¬︎
πŸ“…︎ May 15 2020
🚨︎ report
Minoxidil acts as an antiandrogen: A study of 5Ξ±-reductase type 2 gene expression in a human keratinocyte cell line. ncbi.nlm.nih.gov/pubmed/3…
πŸ‘︎ 49
πŸ“°︎ r/tressless
πŸ’¬︎
πŸ‘€︎ u/exz
πŸ“…︎ Sep 27 2019
🚨︎ report
Level of contact hypersensitivity response to diphencyprone and keratinocyte cancer onlinelibrary.wiley.com/d…
πŸ‘︎ 16
πŸ“°︎ r/science
πŸ’¬︎
πŸ‘€︎ u/HPSoyer
πŸ“…︎ Apr 19 2020
🚨︎ report
Fatty Acid Potassium Had Beneficial Bactericidal Effects and Removed Staphylococcus aureus Biofilms while Exhibiting Reduced Cytotoxicity towards Mouse Fibroblasts and Human Keratinocytes (2019) ncbi.nlm.nih.gov/pubmed/3…
πŸ‘︎ 2
πŸ“°︎ r/ScienceNerds
πŸ’¬︎
πŸ‘€︎ u/Ohioz
πŸ“…︎ Feb 15 2020
🚨︎ report
[Misc] Is promoting proliferation and differentiation of keratinocytes both beneficial to normal skin?
πŸ‘︎ 4
πŸ’¬︎
πŸ‘€︎ u/netok
πŸ“…︎ Nov 17 2019
🚨︎ report
Stunning video of a live Keratinocyte and its organelles at super resolution v.redd.it/wkv405m6jxl31
πŸ‘︎ 105
πŸ“°︎ r/medizzy
πŸ’¬︎
πŸ‘€︎ u/Surgeox
πŸ“…︎ Sep 12 2019
🚨︎ report
Enhanced glycogen metabolism supports the survival and proliferation of HPV-infected keratinocytes in condylomata acuminata sciencedirect.com/science…
πŸ‘︎ 3
πŸ“°︎ r/HPV
πŸ’¬︎
πŸ‘€︎ u/xdhpv
πŸ“…︎ Feb 01 2020
🚨︎ report
Your biggest organ: these are human skin cells, they protect you from the world and make you look beautiful (source and 3 more movies here https://nanolive.ch/promocell-keratinocytes/) gfycat.com/GenerousJealou…
πŸ‘︎ 11
πŸ’¬︎
πŸ‘€︎ u/alexgjones
πŸ“…︎ Mar 20 2019
🚨︎ report
Human Keratinocytes (HaCat) stained for excluding junctions, actin and nuclear counter stain.
πŸ‘︎ 28
πŸ“°︎ r/MicroPorn
πŸ’¬︎
πŸ‘€︎ u/DukeDaquiri
πŸ“…︎ Jun 11 2019
🚨︎ report
ERK activating peptide, AES16-2M promotes wound healing through accelerating migration of keratinocytes nature.com/articles/s4159…
πŸ‘︎ 7
πŸ“°︎ r/Peptides
πŸ’¬︎
πŸ‘€︎ u/Polynomality
πŸ“…︎ Mar 17 2019
🚨︎ report
What do keratinocytes have to do with keratin?

Hi, I recently started teaching myself biology because I'm doing it in my first year of university (next year), and I've never taken it before (applied math/physics major), so I'm sorry if this is a dumb/easy question.

Where is the keratin in keratinocytes? Do keratinocytes secrete keratin? Is keratin highly concentrated in their membrane (like an integral protein)? Does the keratin provide internal support like a cytoskeleton? Or does keratin help make up the ground substance of the extracellular matrix around them?

Please let me know if I've got any major conceptual flaws here, haha.

πŸ‘︎ 2
πŸ“°︎ r/biology
πŸ’¬︎
πŸ‘€︎ u/xandergawsome
πŸ“…︎ Jun 13 2019
🚨︎ report
A fully human system to cultivate skin cells for grafting - Breakthrough research to culture human skin cells called keratinocytes to produce skin grafts has been published by a team of researchers in Nature Communications. duke-nus.edu.sg/news/full…
πŸ‘︎ 77
πŸ“°︎ r/Futurology
πŸ’¬︎
πŸ‘€︎ u/mvea
πŸ“…︎ Nov 01 2018
🚨︎ report
Trametes versicolor (Turkey Tail) polysaccharopeptides (PSP) protect human skin keratinocyte against AAPH-induced oxidative stress (April 2019 research paper)

In vitro research, investigating the protective potential of proteo-glycans (aka PSP, a beta-glucan linked to a peptide, which are said to be the main bioactive ingredients in Turkey Tail) . Quite technical.

The conclusion was that PSP, a natural antioxidant, may be effective for attenuating free radical‐induced oxidative damage in human skin.

Full paper is here.

Also see this related research.

πŸ‘︎ 4
πŸ’¬︎
πŸ‘€︎ u/Kostya93
πŸ“…︎ May 06 2019
🚨︎ report
Your biggest organ: these are human skin cells, they protect you from the world and make you look beautiful (https://nanolive.ch/promocell-keratinocytes/) v.redd.it/q5chrf3oyan21
πŸ‘︎ 6
πŸ“°︎ r/LivingCells
πŸ’¬︎
πŸ‘€︎ u/alexgjones
πŸ“…︎ Mar 20 2019
🚨︎ report
Oncotarget | UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3Οƒ axis inhibits keratinocyte survival and proliferation impactjournals.com/oncota…
πŸ‘︎ 5
πŸ“°︎ r/science
πŸ’¬︎
πŸ‘€︎ u/Oncotarget
πŸ“…︎ Nov 03 2017
🚨︎ report
A few questions regarding establishment of primary keratinocyte cultures from human tissue.

So our lab will be receiving some patient skin tissues soon that I will need to establish, subculture, and cyro for future work. Since I only have experience with pre-established cultures of primary keratinocytes I had a few questions I was hoping someone could answer. My PI is not a biologist (medicinal chemist) and none of my committee members have established primary keratinocytes from tissue before. These are rare and precious samples so I cannot afford to lose them with mistakes, I was actually thinking of practicing a few times on some of my skin (maybe just a few hair follicles) just so I know it will work before I handle the patient tissues.

I am using this protocol as a general guide to the process.

-The protocol calls for use of coating matrix in the flasks seeded with the keratinocytes, however I have never used coating matrix in the past as we use t75 flasks that already allow for cell adhesion (for pre-established keratinocyte culture). Is this step only for labs using flasks that do not facilitate adhesion without addition of the matrix or is there another reason to use this in this situation even if we have adhesive flasks?

-If we also want to collect and sulture the fibroblasts from this same tissue the protocol recommends following this guide, however that protocol specifically calls for neonatal tissue and we will be using adult tissue. Is there any reason I cannot follow this protocol for adult tissue? What differences are there when isolating adult fibroblasts rather than neonatal? The protocol mentions for adult tissue to use more tissue and increase collagenase but how much should I increase this by?

-Recombinant Trypsin/EDTA vs "regular" TE? What is the difference and should it matter? Can I just use the TE I have used in the past?

-What is the difference in using animal-origin free (S7) and animal product containing (EDGS) supplement and how would I decide which to use? We have previously used [HKGS](https://www.t

... keep reading on reddit ➑

πŸ‘︎ 3
πŸ“°︎ r/labrats
πŸ’¬︎
πŸ“…︎ Jun 10 2016
🚨︎ report
Keratinocytes can modulate and directly initiate nociceptive responses elifesciences.org/content…
πŸ‘︎ 10
πŸ“°︎ r/neuroscience
πŸ’¬︎
πŸ‘€︎ u/burtzev
πŸ“…︎ Sep 22 2015
🚨︎ report
Identification of C/EBPΞ± as a novel target of the HPV8 E6 protein regulating miR-203 in human keratinocytes journals.plos.org/plosone…
πŸ‘︎ 2
πŸ“°︎ r/citral
πŸ’¬︎
πŸ‘€︎ u/officialcitral
πŸ“…︎ Jun 22 2017
🚨︎ report
Unsaturated Fatty Acids (such as Oleic FA) Can Induce Calcium Influx into Keratinocytes and Cause Abnormal Differentiation of the Epidermis - What does this mean for topical oils? nature.com/jid/journal/v1…
πŸ‘︎ 5
πŸ’¬︎
πŸ‘€︎ u/ganbattelilone
πŸ“…︎ Aug 19 2014
🚨︎ report
Activation of DNA damage repair pathways in response to nitrogen mustard-induced DNA damage and toxicity in skin keratinocytes [Mutat Res., May 2014 β€” free full-text as author manuscript] ncbi.nlm.nih.gov/pmc/arti…
πŸ‘︎ 3
πŸ“°︎ r/CBRNE
πŸ’¬︎
πŸ‘€︎ u/isosafrole
πŸ“…︎ Jul 08 2015
🚨︎ report
[J] [Mobile phones: 5G] [Mitochondria Dysfunction] Additive Effects of Millimeter Waves and 2-Deoxyglucose Co-Exposure on the Human Keratinocyte Transcriptome ncbi.nlm.nih.gov/pmc/arti…
πŸ‘︎ 2
πŸ“°︎ r/Electromagnetics
πŸ’¬︎
πŸ‘€︎ u/badbiosvictim1
πŸ“…︎ Oct 10 2016
🚨︎ report
[Request] Cannabinoids inhibit human keratinocyte proliferation through a non-CB1/CB2 mechanism and have a potential therapeutic value in the treatment of psoriasis.

Cannabinoids inhibit human keratinocyte proliferation through a non-CB1/CB2 mechanism and have a potential therapeutic value in the treatment of psoriasis.

AuthorsWilkinson JD, et al. Show all Journal J Dermatol Sci. 2007 Feb;45(2):87-92. Epub 2006 Dec 6.

http://www.ncbi.nlm.nih.gov/m/pubmed/17157480/

πŸ‘︎ 5
πŸ“°︎ r/Scholar
πŸ’¬︎
πŸ‘€︎ u/analytical360
πŸ“…︎ Oct 30 2013
🚨︎ report
Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22 -Micro RNA and the genetics of hair loss journals.plos.org/plosgen…
πŸ‘︎ 3
πŸ“°︎ r/science
πŸ’¬︎
πŸ‘€︎ u/burtzev
πŸ“…︎ Sep 29 2015
🚨︎ report
MicroRNA and the genetics of hair loss: Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22 journals.plos.org/plosgen…
πŸ‘︎ 8
πŸ“°︎ r/genetics
πŸ’¬︎
πŸ‘€︎ u/burtzev
πŸ“…︎ Sep 29 2015
🚨︎ report

Please note that this site uses cookies to personalise content and adverts, to provide social media features, and to analyse web traffic. Click here for more information.