I am thinking of making some Girl Scout inspired achievement patches, but for learning lab work. I remember being so disheartened by all the mistakes I made when I was just starting out, that it took me a long time to realize that it was a perfectly normal part of becoming a competent researcher.
What are some of the common mistakes you made early on, that you laugh at now, but had you questioning your ability to do science when you first began?
Here are a few of mine:- Added loading buffer as the ladder in an SDS-PAGE- Making a 0.1% or a 10% agarose gel instead of 1%, or any order of magnitude slip-up- Mixing up MnCl2 and MgCl2 in a buffer/media recipe- Forgetting to add glucose/carbon source to a shake flask experiment- Forgetting to label your tubes and then spilling them- Throwing out the supernatant in a mini-prep when you should have kept it- Putting a vacuum filter on upside down and aspirating off the liquid you needed to sterilize- Not balancing a centrifuge correctly and thinking you're all going to die when it slows down- Getting stuck in buffer pHing purgatory for 2 hours- Spilling your entire freezer box and then finding an important tube under the freezer a year later- Forgetting to add antibiotic to your plates and getting way too many colonies after a transformation
Edit: Wow, thanks so much for everyone's responses! Definitely a trip down memory lane for a lot of these mistakes. Would anyone be interested if I made some patches to commemorate these things? Might be fun gifts for undergrads in the lab. I would donate part of the profits to a STEM education charity/program. Let me know!
I see we have a lot of new members who got duped by the Dogecoin pump and dump. I'm very sorry to see this happen, but props to you all for sticking around. trust me.. it will be worth it.
I made my first bitcoin purchase about 3 years ago. February 2018. I think bitcoin was right around 12,000. If you dont know, it continued to decline for the next year, down to about 3k. I lost 75% of that initial investment, but I continued buying on the way down, and on the way back up. I'll never stop buying bitcoin.
WSB, MOON and HOLD: I'm not trying to take props for anything, but I think the cryptocurrency community coined these terms. We are the original diamond fists. I HODL (hold on for dear life) my coin, and I still own the very first lil chunk of bitcoin I've ever bought.
I hope you guys are experiencing what I felt when I found crypto in 2018, and if you have any questions feel free to ask. My PMs are open
I don’t endorse monk mode for younger practitioners of MGTOW and I support the right to fail.
I am of the impression that MGTOW really is a philosophy and not everyone comes to this philosophy for the same reasons or at the same points in their life.
For some they are ready to embrace an aesthetic lifestyle that a Shaolin monk would approve of, and for others it enables them to step away from the CC long enough to clear their headspace before jumping back in.
I don’t really assume that everyone has the same goals either as we’re all at different stages in our lives. What works for a 40 year old man who has been through the financial bleed out post-divorce isn’t going to work for a 20 year old who hasn’t had a lot of male role models in his life or has received any positive messages about manhood and masculinity from feminist pop culture, a lack of positive male role models or the indoctrination centers they call public schools.
And even though I am an old man I still remember what it was like to be young.
Manhood is a rite of passage. It’s not like that with women who have their periods and that’s the end of it. There is always a testing process, ritual or experience that enables the passage into manhood and in this society too many have not been allowed the rite.
I am of the belief that too many male adults are not actually men. I don’t blame them because we live in a gynocentric culture. At the same time I won’t discourage these man children from interaction with women. For better or worse and imho they need to learn to master their relationships in life or be mastered by them and women are no exception.
And with men like this all I hope to do is impart advice upon them so they don’t get victimized through cohabitation, marriage, or a society that believes all women. A man in this society is literally one accusation away from having his life destroyed.
But that’s the world and if the men of the future are going to master it they’ll need to master themselves and how they deal with the women in their lives either by necessity or choice.
And in light of how this is a philosophy and not a movement I wouldn’t deny anyone the choices I made in life even if I know they are bad. Because even though there is consensus here on female nature sometimes lessons have to be learned through failure.
The most fucked up thing about this society is that we don’t allow people to fail anymore. We give out participation trophies and tell ourselves that we w... keep reading on reddit ➡
Drew explained that every demigod of Aphrodite should complete the goddess’ rite of passage to become worthy of their mother. What if Aphrodite’s Rite of Passage also have a curse when an Aphrodite demigod failed to complete this tradition? Silena refused to do this rite of passage and she died along with Charles. Drew also commented that Silena got her fate for not completing this tradition. Piper also refused to do this and her boyfriend Jason was killed.
We see in PJO that Aphrodite tends to put others in a very dangerous circumstances for the name of love and romance. She even tell Percy that she’ll have more surprise in his quest to save Annabeth which means she made his quest more difficult for she finds it more romantic. Percy himself commented about Aphrodite’s involvement in the Trojan war.
Hello reddit, I grew up in the city and my friend grew up on the coast and we are having a debate about belly button rings. This is clearly an urgent matter to be settled
My opinion as a city slicker- in high school, you knew a girl was either a bogan or a skank if she had a belly button ring (usually combination of both).
My friend, who grew up coastal believes it’s something everyone got between grade 7 and 10 unless your parents were mean and wouldn’t let you get it. And that a belly button ring is a “rite of passage” for a teenage girl.
So reddit, what do you think of the trend of teenage girls getting belly button rings? Is it bogan or am I surprisingly snobby for someone who went to a middle class public school filled with tradie parents?
Other relevant information: we graduated in 2010. I feel like getting it in like the late 90s to 2004 is slightly less yuck because billabong was fashun then.
Growing up I never had lego, I always wanted some and I would ask for it almost every Christmas but I never got it (I think it was too messy a toy for my parents).
I have been looking forward to when my LO is old enough to play with lego. He's only 14 months old at the moment so we're still not there yet, however, for Christmas he got some Duplo. We've been playing with it today and it was a lot of fun.
This evening, during the process of getting him ready for bed I was walking to the door and had a sudden pain in the arch of my foot. I looked down and saw a rogue Duplo block on the floor - I stepped on my first (almost) lego! I'm sure this will be the first of many, and future times will likely not be met with the same pained smile, but today I feel like I've gone through some sort of initiation or rite of passage :)
I feel like rites of passage are often really vague for people voted out pre-merge, but can any of you guys think of a time where they were downright mean or unflattering? i feel like caryn’s was interesting in palau if i remember correctly.
I was spending large chunks of time coming up with batches of info the party could use at the bathhouse and at trivia night. The group was incredible, talking in-character while I scrambled to organize a list of trivia questions, other teams, and more in a way that would hopefully be fun.
Absolutely none of my notes were used in this session, but I tried to use the opportunities to offer bits of lore I've squirreled away ("This fabled sea-dwelling arcanophage, when large enough, can completely sever a region's ties to The Weave": "Kraken", and "The Dernian Goblin Wars met their end after a well-timed wall of fire proved that these creatures made terrible steeds": "Dinosaurs"), all while spacing them out with fill-in-the-blank answers where the party could be silly, joke with other tables, and have a good time ("None of us here are alchemists, but how would you make an Oil of Slipperiness?").
Every session before this felt like a losing battle against the party's antics, with my only weapon being the prepared notes. With this session starting off the rails (The party split up in the first five minutes), I abandoned my preparation (hopefully, only until next time) and tried to make the best of the situation. It wasn't perfect by any stretch, but everybody was laughing, and I felt like my skills as a DM improved by the end of the session.
Perma-banned from r/Coronavirus !!! But I was shooting too high by suggesting that the elites created the pandemic.